The temporary space in Gardner Street is called @SussexUni and will close on 29 May, after being open for a month.
The shop is a pilot scheme and will potentially reappear in the future.
Commenting on the project, John Duffy, Registrar for the University, said: “There are a lot of great things going on at the University and the shop gives us an opportunity to showcase our high-quality research, our range of courses and some brilliant student work.”
The shop has held evening events for teachers and prospective students.
A University spokesperson said:
“We’ve been extremely pleased with the response we’ve had to the pop-up shop.
“Over the last month we’ve had a huge range of people visiting us, from prospective students and their parents, local councillors and businesses to teachers, as well as many of our own students who’ve come in to show their support.
“Our evening events have been very well attended and it’s been great to help people find out more about the university and what’s on offer.
“We’re particularly proud of our student ambassadors who have been staffing the shop, alongside some of our university staff, as they have really shown what Sussex is all about.”